In Vitro Anthelmintic Evaluation of Kudarkirumi Naasini Chooranam by Earthworm Motility Assay (Eudrilus Eugeniae)

Abstract

Kudarkirumi Naasini Chooranam is a classical Siddha polyherbal formulation traditionally used for the management of intestinal helminthic infestations. The present study was undertaken to evaluate the in vitro anthelmintic activity of this formulation using the earthworm motility assay with Eudrilus eugeniae as the experimental model. The formulation was prepared according to standard Siddha procedures and tested at different concentrations to assess its biological activity. The anthelmintic activity was evaluated by recording the time taken for paralysis and death of the earthworms after exposure to the test solutions. The results demonstrated a concentration-dependent effect, with higher concentrations producing a faster onset of paralysis followed by death of the worms. The observed activity is likely due to the interference of active constituents with the neuromuscular coordination and physiological functions of the parasites. The findings of this study indicate that Kudarkirumi Naasini Chooranam possesses significant anthelmintic potential and supports its traditional use in the management of intestinal worms.