Standardization and Quality Evaluation of the Siddha Herbal Formulation Seviya Vadagam (SV) Through Spectroscopic and Chromatographic Techniques

Abstract

Seviya Vadagam (SV) is a traditional Siddha medicine widely indicated for the management of jaundice and hepatic disorders. Although its clinical use is well established, scientific validation using advanced instrumental techniques is essential to define quality control standards. The present study aimed to standardize SV using High-Performance Thin-Layer Chromatography (HPTLC), Fourier Transform Infrared Spectroscopy (FTIR) and Inductively Coupled Plasma Optical Emission Spectroscopy (ICPOES). HPTLC fingerprint profiling revealed a distinct fingerprint pattern for the formulation, ensuring reproducibility and authentication. FT-IR analysis identified the presence of characteristic functional groups corresponding to phytoconstituents. ICP-OES analysis demonstrated that arsenic, cadmium, and mercury were below the limit of quantification, while lead content remained within the permissible safety limits prescribed by regulatory standards. The findings collectively establish instrumental quality parameters for Seviya Vadagam and scientifically support its safety, authenticity, and potential therapeutic application in liver-related disorders while facilitating future pharmacological and clinical research.