Pharmacognostic and Analytical Profiling of Timirahara Lauha Capsule: An Ayurvedic Herbo-Mineral Formulation

Abstract

Timirahara Lauha is a classical Ayurvedic polyherbomineral formulation primarily indicated in the management of Timira- a condition analogous to early-stage of visual impairment, including refractive errors and degenerative ocular changes. The formulation is composed of Lauha Bhasma (Bhasma) along with potent Rasayana (rejuvenative) herbs like Amalaki (Emblica officinalis Linn), Bibhitaki (Terminalia bellirica Roxb.), Haritaki (Terminalia Chebula Ritz.), Yashtimadhu (Glycyrrhiza glabra Linn.) and Padma (Nelumbium speciosum Willd.). This study aims to ensure its quality and efficacy. Material and Methods: The study included the authentication of raw ingredients and the preparation of Timirahara Lauha capsule. The prepared drug was evaluated for organoleptic, physico-chemical, heavy metal content, microbial analysis, mycotoxins and High-Performance Thin-Layer Chromatography (HPTLC) at Quality control laboratory of the All India Institute of Ayurveda (AIIA) and ARBRO pharmaceuticals Pvt. Ltd., New Delhi. Results: Physico-chemical parameters showed loss on drying (3.85%), total ash (47.65%), acid-insoluble ash (41.45%), water-soluble ash (1.00%), water-soluble extractive (22.31%) and alcohol-soluble extractive (12.87%). pH (5% aqueous solution) was 3.84, with a uniformity of weight of 249.8mg and disintegration time of 18 min. HPTLC fingerprinting showed distinct bands at 254nm, 366nm and after derivatization at 540nm with characteristic Rf values. Heavy metal content, aflatoxins, and microbial load were below permissible limits. Gallic acid content was 7.72% w/w. Discussion: The analytical findings confirm the formulation’s compliance with pharmacopoeial standards. The absence of contaminants and defined HPTLC profile support its quality and authenticity. High ash content aligns with the presence of Lauha Bhasma.