@article{Deraniyagala Srianthie_Deraniyagala Nilusha Udayangani_Hettiarachchi Chamari_2020, title={ANTIOXIDANT, ANTIBACTERIAL AND ANTI-INFLAMMATORY POTENTIAL OF THE AQUEOUS EXTRACT OF THE RAW LEAVES OF SRI LANKAN VARIETY OF PERSEA AMERICANA MILLER (AVOCADO)}, volume={8}, url={https://ijapr.in/index.php/ijapr/article/view/1403}, abstractNote={<p>Despite the advances in modern medicine, plant based remedies are currently sought after as alternatives to synthetic drugs. As such natural sources are being searched to use as antioxidants, anti-inflammatory, and antibacterial compounds. This study was focused on the investigation of aqueous leaf extract of Sri Lankan variety of <em>Persea americana </em>(ALEPA) which was prepared according to the method of <em>Kasaya</em> (Decoction) in Ayurvedic medicine to test its antioxidant, anti-inflammatory and antibacterial activities.</p> <p>The total phenolic content of ALEPA was determined by Folin Ciocalteu assay was 681±6.8 mg (PGE) /g (PGE= Pyrogallol equivalents) and the flavonoid content was 1193±302 mg (QE)/g (QE= Quercetin equivalents) according to the AlCl<sub>3</sub> colorimetric assay.</p> <p>DPPH radical scavenging activity (RSA) of ALEPA was 11.3±2.8-52.3±5.0% for concentrations of 50-500 µg/ml whereas ascorbic acid showed 28.0±0.7-90.7±0.1% in the concentration range of 200-500 µg/ml. ALEPA showed better ferrous ion reducing activity compared to ascorbic acid. Hydroxyl RSA of ALEPA was 17.4±1.3-41.5±0.5% in the concentration range of 20-50mg/ml whereas ascorbic acid showed 7.0±0.2-19.8±0.5% for the same concentration range.</p> <p>Overproduction of nitric oxide free radicals are implicated in inflammation. In the NO scavenging assay, ALEPA showed activity 26.0±3.6-69.2±2.5% in the concentrations of 0.2-8 ppm whereas ascorbic acid showed activity 26.8±2.5-49.3±1.9% in the concentrations of 0.2-4 ppm. In human red blood cell (HRBC) assay, ALEPA showed 48.0±1.9-60.6±2.6% HRBC membrane protection whereas the Asprin showed 39.7±0.4-49.0±0.5% activity. The prevention of HRBC membrane lysis is taken as a measure of anti-inflammatory activity and also ALEPA showed antibacterial activity towards <em>Escherichia coli, </em><em>Streptococcus aureus</em><em>, </em>and <em>Bacillus subtilis.</em></p&gt;}, number={3}, journal={International Journal of Ayurveda and Pharma Research}, author={Deraniyagala Srianthie and Deraniyagala Nilusha Udayangani and Hettiarachchi Chamari}, year={2020}, month={Apr.}, pages={1-11} }